The aim of this study was to evaluate the autism awareness among religious officials working in the mosques of a district on the Anatolian side of Istanbul. In this cross-sectional study, a survey was administered to 57 individuals who agreed to participate out of the total number of imams and muezzins working in the mosques of a district on the Anatolian side of Istanbul (n = 93). In this study, 96.5% of the participants (n = 55) stated that they had heard the word “autism” before. About 38.6% of the participants (n = 22) correctly described autism, stating that “The most basic characteristic is social interaction problems.” The percentage of participants stating that ‘Individuals with autism have difficulty expressing themselves” was 73.7% (n = 42). The awareness scores of participants who were muezzins, compared to imams, were statistically significantly higher (p = 0.048). Additionally, the awareness scores of religious officials with individuals/children with autism in their close surroundings were also found to be statistically significantly higher (p = 0.011). The percentage of participants who expressed a willingness to include educational activities in their duties to prevent the social exclusion of individuals with autism was 73.7% (n = 42). Training and seminars for autism awareness and education should emphasize less-known aspects and specific features of autism.